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1.
Rev. colomb. biotecnol ; 15(1): 159-166, ene.-jun. 2013. ilus, tab
Article in Spanish | LILACS | ID: lil-696129

ABSTRACT

Se evalúo el efecto de la salinidad (15, 25 y 35 UPS) y concentración de nitrato (4, 8 y 16 mmoles L-1) sobre el crecimiento y composición bioquímica de la cianobacteria Limnothrix sp. (LAEP- 52) con miras a su explotación para fines biotecnológicos. La cianobacteria se cultivó durante 20 días a 25°C, 98 µmol m-2s-1, fotoperiodo 12:12 y aireación continua (200 mL min-1). El crecimiento fue evaluado cada 48 horas a través de la medición de la densidad óptica a 730 nm. Se evidenció que la salinidad y la concentración de nitrato modulan el crecimiento y la composición bioquímica de Limnothrix sp. El mayor crecimiento (6.3 ± 0.38 mg mL-1), contenidos de proteínas (57 ± 4.56 %), ficocianina (170.3 ± 13.6 µg mL-1) y clorofila a (16 ± 1.28 µg mL-1) se obtuvieron a la menor salinidad (15 UPS) y mayor concentración de nitrato (16 mmoles L-1). Por el contrario, las mayores concentraciones de lípidos (21.3± 1.19 %), carbohidratos (14.47 ± 1.15 %) y carotenoides (6 ± 0.48 µg mL-1) se lograron en la mayor salinidad (35 UPS) y menor concentración de nitrato (4 mmoles L-1). La producción de exopolisacáridos sólo fue influenciada por la salinidad, llegando a alcanzar sus mayores valores a 35 UPS (1600 ± 112.25 mg L-1). Los contenidos de proteínas, lípidos, carbohidratos y pigmentos obtenidos en esta cianobacteria permiten catalogarla como un organismo que puede ser usado en las industrias biotecnológicas, ya sea como alimento para organismos cultivados o como fuente de metabolitos de interés industrial.


In this research we evaluate the effect of salinity (15, 25 and 35 UPS) and nitrate concentration (4, 8 and 16 mmoles L-1) on growth and biochemical composition of the cyanobacterium Limnothrix sp. (LAEP-52) with a view to exploitation for biotechnological purposes. The cyanobacterium was grown in volumes of 1 L for 20 days. The culture conditions included 25 °C, irradiance of 98 µmol m-2 s-1, photoperiod 12:12 and continuous aeration (200 mL min-1). Growth was evaluated every 48 hours through the measurement of optical density at 730 nm. It showed that salinity and concentration of nitrate modulate the growth and biochemical composition of Limnothrix sp. The highest values of growth (6.3 ± 0.38 mg mL-1), protein content (57 ± 4.56%), phycocyanin (170.3 ± 13.6 mg mL-1) and chlorophyll a (16 ± 1.28 mg mL-1) were obtained at the lowest salinity (15 UPS) and highest levels of nitrate (16 mmolesL-1). By contrast, higher concentrations of lipids (21.3 ± 1.19%), carbohydrate (14.47 ± 1.15%) and carotenoids (6 ± 0.48 mg mL-1) were achieved in the highest salinity (35 UPS) and the lowest concentrations of nitrate (4 mmoles L-1). The production of exopolysaccharides was only influenced by salinity, reaching its highest values at 35 UPS (1600 ± 112.25 mg L-1). The content of proteins, lipids, carbohydrates and pigments obtained in this cyanobacterium allow cataloged as an organism which can be used in biotechnology industries, either as feed for farmed organisms or as a source of metabolites of industrial interest.


Subject(s)
Biotechnology , Culture Media , Cyanobacteria , Biochemistry , Chemistry, Bioinorganic
2.
Rev. Fac. Cienc. Méd. (Córdoba) ; 63(1): 18-25, 2006. tab
Article in Spanish | LILACS | ID: lil-472418

ABSTRACT

Con el objeto de cuantificar lo que acontece, entre los integrantes del equipo de salud en el ámbito público y privado de la ciudad de Córdoba, se lleva a cabo la presente investigación. Diseño observacional transversal comparativo sobre muestra estratificada de médicos y fisioterapeutas. La recolección de datos se realizó mediante encuesta de auto-reporte de "Maslach Burnout Inventory" (MBI).Fueron estudiados 479 profesionales, el 22,3%( 107) eran Fisioterapeutas y Kinesiólogos y 77,7% (372) Médicos de diferentes especialidades. Se puede concluir que en el ámbito público, los valores de cansancio emocional se hallan en los rangos publicados, despersonalización y falta de realización personal son menores. Entre los hospitales no difieren significativamente. En el Hospital General del ámbito Provincial, el Burnout es similar al de otros países y superior al de los dos locales. En el ámbito privado los niveles de cansancio emocional son significativamente mayores que en el público, en el Servicio de Emergencia los valores exceden los rangos publicados. Entre los Pre-Residentes, los niveles de estrés son moderados y no parecen variar; el Burnout se hallaría por debajo de los niveles esperados. El estrés promedio de los fisioterapeutas y kinesiólogos se halla en niveles moderados y es menor al de médicos del ámbito público de la ciudad de Córdoba, pero comparable al de los residentes y clínicos. Los especialistas Medicina Legal del Ministerio Público Fiscal, presentan el más alto porcentaje de síndrome de Burnout completo.


With the intention of quantifying what occurs, between the members of the equipment of health in the public and deprived scope of the city of Cordoba, the present investigation is carried out. Comparative cross-sectional design observational on stratified sample of doctors and physiotherapeutic. The data collection was made by means of survey of car-reports of "Maslach Burnout Inventory" (MBI). 479 professionals were studied, 22,3%( 107) they were Physiotherapeutic and Kinesiólogos and 77.7% (372) Doctors of different specialties. It is possible to be concluded that in the public scope, the values of emotional fatigue are in the published ranks, depersonalization and lack of personal accomplishment is smaller. Between the hospitals they do not differ significantly. In the General Hospital of the Provincial scope, the Burnout is similar to the one of other countries and superior to the one of both local. In the private scope the levels of emotional fatigue are significantly greater than in the public, in the Service of Emergency the values exceed the published ranks. Between PreResidentes, the stress levels are moderate and they do not seem to vary; the Burnout would be below the awaited levels. Stress average of physiotherapeutic and kinesiólogos is in moderate levels and is smaller to the one of doctors of the public scope of the city of Cordova, but comparable to the one of the clinical residents and The specialists Legal Medicine of the Fiscal Public Ministry, present/display the highest percentage of syndrome complete Burnout.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Burnout, Professional/epidemiology , Health Personnel , Occupational Health/statistics & numerical data , Argentina/epidemiology , Burnout, Professional/psychology , Cross-Sectional Studies , Emotions , Health Personnel/standards , Health Personnel/statistics & numerical data , Internship and Residency/statistics & numerical data , Private Sector , Public Sector , Physicians/psychology , Sex Factors , Surveys and Questionnaires , Syndrome , Workload
3.
Rev. biol. trop ; 53(3/4): 455-461, sept.-dic. 2005. graf, ilus
Article in English | LILACS | ID: lil-451274

ABSTRACT

We conducted a 5-month experiment at Turpialito in the Golfo de Cariaco, Venezuela, to examine whether the previously reported more rapid growth of scallop Euvola ziczac in bottom compared to suspended culture can be attributed to more abundant or higher quality food resources near the sediment/water interface. The various body components (shell, muscle, digestive gland, gonad and remaining tissues) increased in size at a much greater rate for scallops maintained on the bottom, in partly buried cages at 5 m in depth, than in cages suspended at the same depth in the water column. Furthermore, survival was greater on the bottom. Food abundance and quality were examined by analyzing the seston collected in sediment traps at the sediment/water interface in the vicinity of the bottom cages and next to the suspended cages. Phytoplankton abundance (chlorophyll a) and the proportion of various fatty acids in the lipid fraction of the seston were similar on the bottom and in suspension. However, sestonic protein, lipid and carbohydrate levels, and the estimated energetic content of the seston, were higher on the bottom than in suspension, and probably contributed to the greater growth on the bottom. As the increase in the energetic content of the seston on the bottom compared to in suspension was less than the increase in growth (biomass) on the bottom compared to in suspension, and the evidence showed in previous studies above the negative influence of fouling and wave action in suspended culture, we conclude that the more rapid growth of Euvola ziczac in bottom than suspended culture is principally due to stress relative to suspended culture system


Subject(s)
Animals , Aquaculture/methods , Pectinidae/growth & development , Food Supply , Geologic Sediments/analysis , Organic Chemicals/analysis , Phytoplankton
4.
Rev. biol. trop ; 48(4): 799-806, Dec. 2000.
Article in Spanish | LILACS | ID: lil-320119

ABSTRACT

El efecto de la densidad sobre el crecimiento y la supervivencia de juveniles del pectínido Lyropecten nodosus se estudió en condiciones de cultivo intermedio en el Golfo de Cariaco, Estado Sucre, Venezuela (diciembre 93 - julio 94). Se evaluaron seis densidades de siembra (7, 15, 30, 60, 125 y 250 ejemplares/cesta) con una longitud y una masa seca inicial de 9.4 + 0.18 mm y 0.007 + 0.0026 g, respectivamente. Los juveniles fueron colocados en cestas japonesas "pearl nets" y posteriormente suspendidas en un "long line" a 8 m de profundidad. Los patrones de crecimiento en cada uno de los compartimientos del soma fueron similares, pero con magnitudes diferentes e inversamente proporcionales a la densidades de cultivo; sin embargo, la supervivencia no fue afectada significativamente, sugiriendo que la competencia intraespecífica de los organismos en cultivo provocó un estrés notable, aunque insuficiente para causar mortalidad. Los resultados y estrategias proyectadas para el cultivo intermedio de L. nodosus sugieren un procedimiento de disminución de la densidad de organismos a través de desdobles sucesivos en un período de cultivo de seis meses obteniendo tallas cercanas a los 50 mm para comenzar el cultivo de engorde.


Subject(s)
Animals , Fisheries , Mollusca , Seawater , Population Density , Venezuela
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